Protective Effect of Vitamin b6 On Lead-Induced Hematological Changes in Male Albino Wistar Rats

Inneh C. A and Nzoputam O. J

1Department of Physiology, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria.

Corresponding Author: churchill.inneh@uniben.edu

Recieved Date: 14th March, 2023; Accepted Date: 4th May, 2023; Published Date: June, 2023

Citation: Inneh C. A and Nzoputam O. J

ABSTRACT

Background: Lead is a common environmental pollutant capable of causing acute and chronic illness. Lead acetate has been reported to have a higher binding affinity for erythrocytes and also for inducing haemolysis. This study was carried out to investigate the effect of lead acetate on some hematological parameters and the protective effect of Vitamin B6. Methods: Twenty (20) adult male albino Wistar rats, weighing between 180-250g, were randomly assigned into 4 groups with 5 rats per group. Group A rats served as control. Group B rats were administered 50mg/kg body weight of lead acetate; Group C rats were administered 50mg/kg body weight of vitamin B6; Group D rats were co-administered vitamin B6 and lead acetate. This experiment lasted for 28 days. At the end of the experimental period, the animals were sacrificed and blood samples were collected, by Cardiac puncture, and transferred into EDTA bottles. The hematological parameters were analyzed were Red blood cell (RBC) count, Hemoglobin concentration (HbC), packed cell volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), White Blood Cell (WBC) count, differential white blood cell count (lymphocyte, granulocyte and monocyte) and Platelet count. Statistical analysis was done using graph pad Prism 8.0 software. The results were presented as mean ± SEM and P<0.05 was taken to be statistically significant. Results. There was a significant reduction in RBC, PCV, Hemoglobin concentration, MCV, MCHC, MCH and a significant increase in WBC, lymphocyte and platelet counts in rats administered with lead acetate only. In rats that were co-administered with lead acetate and vitamin B6, there was a significant improvement in RBC, PCV, Hemoglobin concentration, MCV, MCHC, MCH and a significant decrease in WBC, lymphocyte and platelet counts when compared with rats that wereadministered with lead acetate only. Conclusion: In conclusion, this study has shown that vitamin B6 protected the blood cells against lead-induced damage. Therefore it could be an effective therapeutic agent against lead-induced hematological changes and its associated complications