Antibiotic Susceptibility of Contaminated Cheese `Wara` in Owo, Ondo State

1 Oloyede Samuel Bolaji, 1 Olutoyin Catherine Adekunle, 2Akindele Amos Ajayi, 1Abolaji Tolulope Adeyemo, 3Adeola Olajumoke Ajayi, 4Joyce Damilola Odunayo
1Department of Medical Microbiology and Parasitology, Osun State University, Osogbo, Nigeria
2Department of Family Medicine, Osun State University, Osogbo, Nigeria.
3Department of Psychiatry, Osun State University, Osogbo, Nigeria
4Department of Medical Laboratory Sciences, Achievers University, Owo, Nigeria
Corresponding Author: oloyede.bolaji@uniosun.edu.ng; Tel: +2348037900913
Recieved Date: 28th August, 2022; Accepted Date: 12th December, 2022; Published Date: December, 2022
Citation: Bolaji, OS, Adekunle, OC, Ajayi, AA, Adeyemo, AT, Ajayi, AO, Odunayo, JD. Antibiotic Susceptibility of Contaminated Cheese “Wara” in Owo, Ondo State J Basic Appl Med Scis, 2022;2(2):9-15
ABSTRACT

Background: Cheese is one of the products gotten from milk. The manufacturing process of soft cheese in Nigeria is lagging behind and as a result, there is difficulty in production, storage (extending shelf life) and preserving the nutritious components of milk. Food safety is a global concern and must be taken seriously therefore this study was designed to investigate possible bacterial contamination of locally made cheese (Wara) and antibiotic susceptibility pattern of the bacterial isolates. Methodology: A total number of twenty samples of “Wara” were purchased from four different locations in Owo, Ondo State, Nigeria. Bacteriological culture was done using standard methods. The identification of the isolates was based on morphological and biochemical characteristics exhibited by the isolates. Results: Four bacteria were isolated from locally made cheese sold in Owo. They were Citrobacter (20%), Escherichia coli (50%), Enterobacter (15%) and Klebsiella (15%) respectively. The Total viable count of Citrobacter was 6.2 x 108 CFU/ml, Escherichia coli was 4.8 x 108 CFU/ml, Enterobacter was 3.3 x 108 CFU/ml and Klebsiella was 5.8 x 108 CFU/ml respectively. Conclusion: The majority of isolates were susceptible to Imipenem, Ofloxacin, Gentamycin, Levofloxacin and Ceftriaxone, but was resistant to Nitrofuranton. Cefuroxime and Cefexime. There is a need to standardise the production methods so that the cheese will be free from any form of contamination.

Keywords: Cheese, bacterial contamination, antibiotics, culture, isolation
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